NADK Protein (His tag)
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- Target See all NADK Proteins
- NADK (NAD Kinase (NADK))
- Protein Type
- Recombinant
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Origin
- Mycobacterium tuberculosis
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NADK protein is labelled with His tag.
- Purpose
- NAD Kinase (M. tuberculosis) (rec.) (His)
- Characteristics
- NAD kinase (Mycobacterium tuberculosis) is fused at the N-terminus to a His-tag.
- Purity
- >98 % (SDS-PAGE)
- Biological Activity Comment
- ~1.2 U/mg protein. (Substrate: NAD (+ ATP)) ~3.8 U/mg protein. (Substrate: NAD (+ poly(P)) One unit is defined as the amount of enzyme that synthesizes 1μmol nicotinamide adenine dinucleotide phosphate (NADP) per min.
- Top Product
- Discover our top product NADK Protein
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NAD Kinase (NADK) (AA 64-298) protein (His tag)
NADK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
NAD Kinase (NADK) (AA 1-446) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
NAD Kinase (NADK) (AA 1-110) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- 0.5 mg/mL (Lot specific)
- Concentration
- Lot specific
- Buffer
- In 10 mM TRIS at pH 8.0, containing 1 mM MgCl2, 0.5 mM EDTA, 1 mM DTT and 1 mM PMSF.
- Handling Advice
- After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
- Expiry Date
- 6 months
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- Target
- NADK (NAD Kinase (NADK))
- Alternative Name
- NAD Kinase (NADK Products)
- Background
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Probable Inorganic Polyphosphate/ATP-NAD kinase, Poly(P)/ATP NAD Kinase 1, ppnK1, EC 2.7.1.23
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Mycobacterium tuberculosis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target. Catalytic activity: ATP + NAD+ = ADP + NADP+
- Molecular Weight
- ~33.0kDa
- UniProt
- P0A5S6
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