WISP3 Protein (Transcript Variant 1)
WISP3
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Func, AbP, PI, STD
Active
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- Target See all WISP3 Proteins
- WISP3 (Wnt1 Inducible Signaling Pathway Protein 3 (WISP3))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant 1
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human WISP3 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 ug/ml.
- Top Product
- Discover our top product WISP3 Protein
-
Wnt1 Inducible Signaling Pathway Protein 3 (WISP3) (Active) protein
WISP3 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Wnt1 Inducible Signaling Pathway Protein 3 (WISP3) (AA 1-372) protein (GST tag)WISP3 Origin: Human Host: Wheat germ Recombinant AA, AP, ELISA, WB
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- WISP3 (Wnt1 Inducible Signaling Pathway Protein 3 (WISP3))
- Alternative Name
- Wisp3 (WISP3 Products)
- Synonyms
- CCN6 Protein, LIBC Protein, PPAC Protein, PPD Protein, ENSMUSG00000062074 Protein, Gm735 Protein, WISP-3 Protein, RGD1564120 Protein, WISP3 Protein, si:dkey-119m7.6 Protein, WNT1 inducible signaling pathway protein 3 Protein, WISP3 Protein, Wisp3 Protein, wisp3 Protein
- Background
- This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 36.8 kDa
- NCBI Accession
- NP_003871
- Pathways
- WNT Signaling, Growth Factor Binding
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