Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

WISP1 Protein (Transcript Variant 1)

WISP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD, Func, PI Active
Catalog No. ABIN2735599
  • Target See all WISP1 Proteins
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    Transcript Variant 1
    Origin
    • 5
    • 4
    • 1
    Human
    Source
    • 5
    • 3
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
    Specificity
    Optimal preservation of protein structure, post-translational modifications and functions.
    Characteristics
    • Recombinant human WISP1 (transcript variant 1) protein expressed in E. coli.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.
    Purity
    > 95 % as determined by SDS-PAGE and Coomassie blue staining
    Endotoxin Level
    Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
    Biological Activity Comment
    ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 ug/ml.
    Top Product
    Discover our top product WISP1 Protein
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays
    Restrictions
    For Research Use only
  • Buffer
    Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
    Handling Advice
    Resuspend the protein in the desired concentration in proper buffer
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    Alternative Name
    Wisp1 (WISP1 Products)
    Synonyms
    CCN4 Protein, WISP1c Protein, WISP1i Protein, WISP1tc Protein, WISP-1 Protein, WISP1 Protein, si:ch211-38f10.2 Protein, AW146261 Protein, Elm1 Protein, zgc:172276 Protein, WNT1 inducible signaling pathway protein 1 Protein, WNT1 inducible signaling pathway protein 1a Protein, WNT1-inducible-signaling pathway protein 1-like Protein, WNT1 inducible signaling pathway protein 1b Protein, WISP1 Protein, wisp1a Protein, Wisp1 Protein, LOC100627044 Protein, wisp1b Protein
    Background
    This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83 % identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.
    Molecular Weight
    38.1 kDa
    NCBI Accession
    NP_003873
    Pathways
    WNT Signaling, Growth Factor Binding
You are here:
Support