UPF3B Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
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- Target See all UPF3B Proteins
- UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This UPF3B protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human UPF3B / RENT3B (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product UPF3B Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))
- Alternative Name
- Upf3b,rent3b (UPF3B Products)
- Synonyms
- HUPF3B Protein, MRXS14 Protein, RENT3B Protein, UPF3X Protein, 5730594O13Rik Protein, AI317193 Protein, AW541158 Protein, RGD1560264 Protein, UPF3B, regulator of nonsense mediated mRNA decay Protein, UPF3 regulator of nonsense transcripts homolog B (yeast) Protein, UPF3B Protein, Upf3b Protein
- Background
- This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 56 kDa
- NCBI Accession
- NP_075386
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