TIMP1 Protein (Transcript Variant 2) (His tag)
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- Target See all TIMP1 Proteins
- TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant 2
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TIMP1 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human TIMP1 (transcript variant 2) protein expressed in HEK293.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 90 % , as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg of protein as determine by the LAL method.
- Biological Activity Comment
- Mouse TIMP-1 inhibits the activity of activated mouse MMP-9 (100 ng/mL). The IC50 = 90 ng/mL.
- Top Product
- Discover our top product TIMP1 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
- Alternative Name
- Timp1 (TIMP1 Products)
- Synonyms
- TIMP-1 Protein, TIMP1 Protein, DKFZp468A0912 Protein, CLGI Protein, EPA Protein, EPO Protein, HCI Protein, TIMP Protein, Timp Protein, Clgi Protein, TIMP metallopeptidase inhibitor 1 Protein, tissue inhibitor of metalloproteinase 1 Protein, TIMP1 Protein, Timp1 Protein
- Molecular Weight
- 22 kDa
- NCBI Accession
- NP_035723
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