TYMS Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2733654

Product Details

Target: TYMS (Thymidylate Synthetase (TYMS))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TYMS protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Thymidylate synthase (TS) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: Thymidylate Synthase (Ts) (TYMS Products)

Target Type: Viral Protein

Background: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.

Molecular Weight: 35.5 kDa

NCBI Accession: NP_001062

Pathways: Mitotic G1-G1/S Phases