SMAD2 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
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- Target See all SMAD2 Proteins
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SMAD2 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human SMAD2 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product SMAD2 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
- Alternative Name
- Smad2 (SMAD2 Products)
- Synonyms
- JV18 Protein, JV18-1 Protein, MADH2 Protein, MADR2 Protein, hMAD-2 Protein, hSMAD2 Protein, jv18 Protein, Xmad2 Protein, madh2 Protein, madr2 Protein, XSmad2 Protein, hmad-2 Protein, hsmad2 Protein, jv18-1 Protein, smad-2 Protein, fj43c06 Protein, wu:fj43c06 Protein, 7120426M23Rik Protein, Madh2 Protein, Madr2 Protein, Smad-2 Protein, mMad2 Protein, MAD homolog 2 Protein, Mothers against DPP homolog 2 Protein, SMAD 2 Protein, Smad2 Protein, SMAD family member 2 Protein, SMAD family member 2-Z Protein, SMAD family member 2 S homeolog Protein, SMAD2 Protein, SMAD2Z Protein, smad2 Protein, smad2.S Protein, Smad2 Protein
- Background
- The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
- Molecular Weight
- 52.1 kDa
- NCBI Accession
- NP_001003652
- Pathways
- Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
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