Shugoshin Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2731936

Product Details

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Shugoshin protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human SGOL1 / SGO1 (transcript variant B1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Alternative Name: Sgol1,sgo1 (SGOL1 Products)

Synonyms: sgo1 Protein, NY-BR-85 Protein, SGO Protein, Sgo1 Protein, 3300001M08Rik Protein, C81037 Protein, shugoshin 1 Protein, shugoshin centromeric cohesion 1 Protein, shugoshin 1 L homeolog Protein, Sgo1 Protein, sgo1 Protein, sgo1.L Protein, SGO1 Protein

Background: The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants.

Molecular Weight: 31.1 kDa

NCBI Accession: NP_001012411

Pathways: Maintenance of Protein Location