RSPO1 Protein
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- Target See all RSPO1 Proteins
- RSPO1 (R-Spondin 1 (RSPO1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- CHO Cells
- Application
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human RSPO1 protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- R-spondin-1 enhances BMP-2 mediated differentiation of MC3T3-E1 cells. The expected ED50 is 1.0-3.0 ug/ml.
- Top Product
- Discover our top product RSPO1 Protein
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R-Spondin 1 (RSPO1) protein (His tag)
RSPO1 Origin: Human Host: CHO Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
R-Spondin 1 (RSPO1) (AA 21-265) protein (His tag)RSPO1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
R-Spondin 1 (RSPO1) (AA 21-146) protein (His tag)RSPO1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
R-Spondin 1 (RSPO1) (AA 21-146) protein (Fc Tag)RSPO1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
R-Spondin 1 (RSPO1) (AA 21-263) protein (His tag)RSPO1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
R-Spondin 1 (RSPO1) (Active) protein (His tag)RSPO1 Origin: Mouse Host: CHO Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- RSPO1 (R-Spondin 1 (RSPO1))
- Alternative Name
- Rspo1 (RSPO1 Products)
- Synonyms
- RGD1565558 Protein, CRISTIN3 Protein, RSPO Protein, R-spondin Protein, Rspondin Protein, wu:fj81e05 Protein, zgc:92153 Protein, R-spondin 1 Protein, Rspo1 Protein, RSPO1 Protein, LOC100049655 Protein, rspo1 Protein
- Background
- This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 26.7 kDa
- NCBI Accession
- NP_001033722
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