Ribonuclease H1 Protein (RNASEH1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2730997

Product Details

Target: Ribonuclease H1 (RNASEH1)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Ribonuclease H1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human RNASEH1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Ribonuclease H1 (RNASEH1)

Alternative Name: Rnaseh1 (RNASEH1 Products)

Synonyms: H1RNA Protein, RNH1 Protein, 74/9 Protein, CG8729 Protein, Dmel\\CG8729 Protein, RNase H1 Protein, RnH1 Protein, l(2)43F1-5 Protein, l(2)43Fa Protein, l(2)k07409 Protein, l(2)k07624 Protein, rnhl Protein, h1rna Protein, rnh1 Protein, zgc:91971 Protein, RNASEH1P1 Protein, RNASEH1 Protein, rnaseh1 Protein, Afu1g10020 Protein, Tb07.26A24.40 Protein, ribonuclease H1 Protein, ribonuclease H1 S homeolog Protein, ribonuclease H1 L homeolog Protein, ribonuclease HI Protein, Ribonuclease H1 Protein, RNASEH1 Protein, Rnaseh1 Protein, rnh1 Protein, rnaseh1.S Protein, rnaseh1 Protein, rnaseh1.L Protein, AFUA_1G10020 Protein, Tb927.7.4930 Protein, APH_RS00510 Protein

Background: This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which overlaps the first initiation start codon in human. An alternately spliced transcript variant has been found which encodes a shorter isoform. This gene has three pseudogenes two of them are at different locations of chromosome 17 and one of them is on chromosome 1q32.2.

Molecular Weight: 31.9 kDa

NCBI Accession: NP_002927