PDGFB Protein
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- Target See all PDGFB Proteins
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human PDGFB protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 ng/ml.
- Top Product
- Discover our top product PDGFB Protein
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Platelet Derived Growth Factor Subunit B (PDGFB) (Active) protein
PDGFB Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) protein (GST tag)PDGFB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) protein (His tag)PDGFB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) (Active) protein (His tag,AVI tag)PDGFB Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE. Active
Platelet Derived Growth Factor Subunit B (PDGFB) (Transcript Variant 1) (Active) proteinPDGFB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- Alternative Name
- Pdgfb (PDGFB Products)
- Synonyms
- PDGF-2 Protein, PDGF2 Protein, SIS Protein, SSV Protein, c-sis Protein, pdgf2 Protein, sis Protein, si:ch211-79m20.1 Protein, si:dkey-261d11.1 Protein, PDGF-B Protein, Sis Protein, platelet derived growth factor subunit B Protein, platelet-derived growth factor beta polypeptide Protein, platelet derived growth factor subunit B S homeolog Protein, platelet-derived growth factor beta polypeptide a Protein, platelet derived growth factor, B polypeptide Protein, PDGFB Protein, pdgfb.S Protein, pdgfba Protein, Pdgfb Protein
- Molecular Weight
- 24.4 kDa
- NCBI Accession
- NP_035187
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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