PAK4 Protein (Transcript Variant 1)

Catalog No. ABIN2728177

Product Details

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human PAK4 (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 90 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Specific activity was determined as 5,595 pmoles/min/μg, according to the Zlyte assay protocol

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol, 5 mM DTT.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Alternative Name: Pak4 (PAK4 Products)

Synonyms: 5730488L07Rik Protein, AW555722 Protein, mKIAA1142 Protein, im:5916170 Protein, wu:fd12b01 Protein, zgc:92014 Protein, p21 (RAC1) activated kinase 4 Protein, p21 protein (Cdc42/Rac)-activated kinase 4 Protein, PAK4 Protein, Pak4 Protein, pak4 Protein

Background: PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molecular Weight: 33.3 kDa

NCBI Accession: NP_005875

Pathways: RTK Signaling