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NP protein (Myc-DYKDDDDK Tag)

Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN2727496
  • Target
    NP
    Protein Type
    Recombinant
    Origin
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 2
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    Myc-DYKDDDDK Tag
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human NP protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Concentration
    50 μg/mL
    Buffer
    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    NP
    Target Type
    Viral Protein
    Background
    This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.
    Molecular Weight
    31.9 kDa
    NCBI Accession
    NP_000261
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