NMT1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2727416

Product Details

Target: NMT1 (N-Myristoyltransferase 1 (NMT1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This NMT1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human NMT1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: NMT1 (N-Myristoyltransferase 1 (NMT1))

Alternative Name: Nmt1 (NMT1 Products)

Synonyms: NMT Protein, im:2601337 Protein, nmt1 Protein, wu:fc15d01 Protein, wu:fc18a04 Protein, zgc:110714 Protein, MGC145283 Protein, AW536594 Protein, ARABIDOPSIS THALIANA MYRISTOYL-COA:PROTEIN N-MYRISTOYLTRANSFERASE Protein, ATNMT1 Protein, MHM17.15 Protein, MHM17_15 Protein, N-MYRISTOYLTRANSFERASE 1 Protein, myristoyl-CoA:protein N-myristoyltransferase Protein, N-myristoyltransferase 1 Protein, N-myristoyltransferase 1a Protein, N-myristoyltransferase 1 L homeolog Protein, N-myristoyltransferase 1 (predicted) Protein, myristoyl-CoA:protein N-myristoyltransferase Protein, NMT1 Protein, nmt1a Protein, nmt1.L Protein, nmt1 Protein, SPBC2G2.11 Protein, Nmt1 Protein

Background: Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1 MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008].

Molecular Weight: 56.6 kDa

NCBI Accession: NP_066565

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling