Nerve Growth Factor Protein (NGF) (Transcript Variant A)
NGF
Origin: Mouse
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD, Func, PI
Active
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- Target See all Nerve Growth Factor (NGF) Proteins
- Nerve Growth Factor (NGF)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant A
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Application
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human NGF (transcript variant A) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is less than or equal to 1.0 ng/ml, corresponding to a specific activity of > 1 x 10^6 units/mg.
- Top Product
- Discover our top product NGF Protein
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Nerve Growth Factor (NGF) (AA 122-239) protein (His tag)
NGF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nerve Growth Factor (NGF) (AA 200-299) protein (GST tag)NGF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Nerve Growth Factor (NGF)
- Alternative Name
- Ngf (NGF Products)
- Synonyms
- Beta-NGF Protein, HSAN5 Protein, NGFB Protein, Ngfb Protein, ngfb Protein, ns:zft386 Protein, xx:zft386 Protein, zgc:109730 Protein, beta-NGF Protein, hsan5 Protein, beta-ngf Protein, NGF Protein, ngf Protein, nerve growth factor Protein, nerve growth factor b (beta polypeptide) Protein, nerve growth factor S homeolog Protein, NGF Protein, Ngf Protein, ngfb Protein, ngf Protein, ngf.S Protein
- Molecular Weight
- 13.4 kDa
- NCBI Accession
- NP_038637
- Pathways
- Regulation of Cell Size
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