NAGPA Protein (Myc-DYKDDDDK Tag)
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- Target See all NAGPA Proteins
- NAGPA (N-Acetylglucosamine-1-phosphodiester alpha-N-Acetylglucosaminidase (NAGPA))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NAGPA protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human NAGPA protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product NAGPA Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- NAGPA (N-Acetylglucosamine-1-phosphodiester alpha-N-Acetylglucosaminidase (NAGPA))
- Abstract
- NAGPA Products
- Synonyms
- NAGPA Protein, APAA Protein, UCE Protein, AI596180 Protein, N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase Protein, NAGPA Protein, NAEGRDRAFT_56913 Protein, Nagpa Protein
- Background
- Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients.
- Molecular Weight
- 53.3 kDa
- NCBI Accession
- NP_057340
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