MRPL32 Protein (Myc-DYKDDDDK Tag)
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- Target See all MRPL32 products
- MRPL32 (Mitochondrial Ribosomal Protein L32 (MRPL32))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MRPL32 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human MRPL32 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MRPL32 (Mitochondrial Ribosomal Protein L32 (MRPL32))
- Alternative Name
- Mrpl32 (MRPL32 Products)
- Synonyms
- BcDNA:LD41522 Protein, CG12220 Protein, Dmel\\CG12220 Protein, L32 Protein, RpL32 Protein, L32mt Protein, MRP-L32 Protein, bMRP-59b Protein, 0610033O15Rik Protein, GB14551 Protein, zgc:153328 Protein, mitochondrial ribosomal protein L32 Protein, 39S ribosomal protein L32, mitochondrial Protein, mitochondrial 54S ribosomal protein YmL32 Protein, mitochondrial ribosomal protein subunit L32 (predicted) Protein, mitochondrial ribosomal protein L32 L homeolog Protein, mRpL32 Protein, MRPL32 Protein, Mrpl32 Protein, LOC413398 Protein, mrpl32 Protein, CAALFM_CR04580WA Protein, mrpl32.L Protein
- Background
- Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.
- Molecular Weight
- 21.2 kDa
- NCBI Accession
- NP_114109
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