Melanoma gp100 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2725809

Product Details

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Melanoma gp100 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Melanoma gp100 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: Melanoma Gp100 (PMEL Products)

Synonyms: D12S53E Protein, ME20 Protein, ME20-M Protein, ME20M Protein, P1 Protein, P100 Protein, PMEL17 Protein, SI Protein, SIL Protein, SILV Protein, gp100 Protein, D10H12S53E Protein, D12S53Eh Protein, Pmel17 Protein, Si Protein, Silv Protein, gp87 Protein, RPE1 Protein, MMP115 Protein, silverb Protein, silver Protein, cb397 Protein, fdv Protein, pmel17 Protein, sb:cb397 Protein, silva Protein, wu:fc11g11 Protein, wu:fj24g11 Protein, zgc:136622 Protein, premelanosome protein Protein, premelanosome protein b Protein, premelanosome protein a Protein, PMEL Protein, Pmel Protein, pmelb Protein, pmela Protein

Background: This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.

Molecular Weight: 70.1 kDa

NCBI Accession: NP_008859