IL27 Protein
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- Target See all IL27 products
- IL27 (Interleukin-27 Subunit alpha (IL27))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- HEK-293 Cells
- Application
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human Interleukin-27 / IL27A protein expressed in HEK293.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- Purity is > 95 % , as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
- Biological Activity Comment
- The ED50 is 5 - 10 ng/ml, corresponding to a specific activity of 1.0 - 2.0 x 10^5 units/mg, determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 20 mM NaH2PO4, pH 6, 0.60 M NaCl
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- IL27 (Interleukin-27 Subunit alpha (IL27))
- Alternative Name
- Interleukin-27,il27a (IL27 Products)
- Synonyms
- IL-27 Protein, IL-27A Protein, IL27A Protein, IL27p28 Protein, IL30 Protein, p28 Protein, EUROIMAGE1875335 Protein, IL25 Protein, IL27 Protein, IL27w Protein, R33729_1 Protein, SF20 Protein, IL-27p28 Protein, Il30 Protein, interleukin 27 Protein, myeloid derived growth factor Protein, IL27 Protein, MYDGF Protein, Il27 Protein
- Molecular Weight
- 49 kDa
- NCBI Accession
- NP_663611
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