Histone H4 Protein (His tag)

Catalog No. ABIN2722826

Product Details

Target: Histone H4

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Histone H4 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Histone H4 (full length, N-term HIS tag) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Histone H4

Abstract: Histone H4 Products

Synonyms: hist1h4l Protein, H1ft Protein, Hist4 Protein, Hist4h4 Protein, H4 Protein, X04652 Protein, FO108 Protein, H4/n Protein, H4F2 Protein, H4FN Protein, HIST2H4 Protein, H4C13 Protein, H4C7 Protein, F8N16.2 Protein, F8N16_2 Protein, histone H4 Protein, BcDNA:RH52884 Protein, CG3379 Protein, Dmel\CG3379 Protein, H4r Protein, His4-88CD Protein, His4R Protein, histone cluster 1, H4a L homeolog Protein, histone cluster 1 H4 family member B Protein, histone cluster 2, H4 Protein, histone cluster 2 H4 family member a Protein, histone H4 Protein, Histone H4 replacement Protein, Histone H4 Protein, hist1h4a.L Protein, Hist1h4b Protein, Hist2h4 Protein, HIST2H4A Protein, H4 Protein, H4C14 Protein, HIS4 Protein, HIST2H4 Protein, His4r Protein, his-38 Protein

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated this record represents the centromeric copy.

Molecular Weight: 11.2 kDa

NCBI Accession: NP_003539