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Histone H4 Protein (Myc-DYKDDDDK Tag)

Name: Histone H4 Protein (Myc-DYKDDDDK Tag)
SKU: ABIN2722824
Price: 956.4 USD
Availability: InStock

Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD

Validation with Western Blot (Histone H4 Protein (Myc-DYKDDDDK Tag))

1 image

Catalog No. ABIN2722824

Datasheet as PDF

Target See all Histone H4 Proteins

Histone H4

Protein Type

Recombinant
Origin
8

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Human
Source
38

11
HEK-293 Cells

Purification tag / Conjugate

This Histone H4 protein is labelled with Myc-DYKDDDDK Tag.

Application

Antibody Production (AbP), Standard (STD)

Characteristics
- Recombinant human Histone H4 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
Purity

> 80 % as determined by SDS-PAGE and Coomassie blue staining

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Histone H4 (AA 2-103) protein (His tag,GST tag)
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Histone H4 protein (His tag)
Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD

Histone H4 (3meLys20) protein
Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant The recombinant histone is >98 % pure by SDS-PAGE. PC, S

Histone H4 (3meLys16) protein
Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant The recombinant histone is >98 % pure by SDS-PAGE. PC, S

Histone H4 (meLys16) protein
Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant The recombinant histone is >98 % pure by SDS-PAGE. PC, S

Histone H4 (2meLys16) protein
Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant The recombinant histone is >98 % pure by SDS-PAGE. PC, S

Histone H4 (3meLys5) protein
Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant The recombinant histone is >98 % pure by SDS-PAGE. PC, S

Histone H4 (2meLys5) protein
Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant The recombinant histone is >98 % pure by SDS-PAGE. PC, S

Histone H4 (meLys5) protein
Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant The recombinant histone is >98 % pure by SDS-PAGE. PC, S

Application Notes

Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment

The tag is located at the C-terminal.

Restrictions

For Research Use only
Concentration

50 μg/mL

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage

-80 °C

Storage Comment

Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Target

Histone H4

Abstract

Histone H4 Products

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails instead, they contain a palindromic termination element.

Molecular Weight

11.2 kDa

NCBI Accession

NP_778224