Histone H3.3 Protein (His tag)

Catalog No. ABIN2722821

Product Details

Target: Histone H3.3

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Histone H3.3 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Histone H3.3 (full length, N-term HIS tag) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 100 mM Arg. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Histone H3.3

Abstract: Histone H3.3 Products

Synonyms: H3.3A Protein, H3F3 Protein, H3-IX Protein, H3-X Protein, H3.3B Protein, H3F3C Protein, H3F3B Protein, H3 histone family member 3A Protein, H3 histone, family 3A Protein, H3 histone, family 3B Protein, H3 histone, family 3C L homeolog Protein, histone H3.3 Protein, Histone H3.3 Protein, histone H3.3B-like Protein, H3F3A Protein, H3F3B Protein, H3f3a Protein, h3f3c.L Protein, LOC5567559 Protein, Bm1_29875 Protein, Bm1_40030 Protein, PTRG_03457 Protein, Phum_PHUM492720 Protein, UREG_07214 Protein, PAAG_07099 Protein, PITG_06950 Protein, PITG_06953 Protein, PITG_06955 Protein, LOC100361558 Protein, LOC100521680 Protein

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17.

Molecular Weight: 15.3 kDa

NCBI Accession: NP_005315