Histone H3.3 Protein (His tag)
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- Target See all Histone H3.3 Proteins
- Histone H3.3
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Histone H3.3 protein is labelled with His tag.
- Application
- Standard (STD), Antibody Production (AbP)
- Characteristics
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- Recombinant human Histone H3.3 (full length, N-term HIS tag) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product Histone H3.3 Protein
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Histone H3.3 (full length ) protein
Origin: Human Host: Escherichia coli (E. coli) Recombinant EAA, ScA
Histone H3.3 (biotinylated), (C-Term), (full length) proteinOrigin: Human Host: Escherichia coli (E. coli) Recombinant EAA, ScA
Histone H3.3 (AA 2-136) protein (His-SUMO Tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Histone H3.3 (AA 2-136), (full length) protein (GST tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant 95 % ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 100 mM Arg. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Histone H3.3
- Abstract
- Histone H3.3 Products
- Background
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17.
- Molecular Weight
- 15.3 kDa
- NCBI Accession
- NP_005315
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