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Histone H3.2 protein (His tag)

Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining STD, AbP
Catalog No. ABIN2722819
  • Target
    Histone H3.2
    Protein Type
    Recombinant
    Origin
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
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    Human
    Source
    • 13
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    His tag
    Application
    Standard (STD), Antibody Production (AbP)
    Characteristics
    • Recombinant human Histone H3.2 (N-term HIS tag) protein expressed in E. coli.
    • Produced with end-sequenced ORF clone
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the N-terminal.

    Restrictions
    For Research Use only
  • Concentration
    50 μg/mL
    Buffer
    25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    Histone H3.2
    Synonyms
    H3-I Protein, H3-II Protein, H3-III Protein, H3-IV Protein, H3-V Protein, H3-VI Protein, H3-VII Protein, H3-VIII Protein, H3F3A Protein, H3C2 Protein, H3C3 Protein, H3 histone, family 3C Protein, histone H3.2 Protein, Histone H3.2 Protein, H3F3C Protein, LOC504599 Protein, PVX_082615 Protein, Tsp_16072 Protein, H3C4 Protein, LOC106362349 Protein
    Background
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated this record represents the telomeric copy.
    Molecular Weight
    15.2 kDa
    NCBI Accession
    NP_066403
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