Histone H3.2 protein (His tag)
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
STD, AbP
1 image
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- Target
- Histone H3.2
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- Standard (STD), Antibody Production (AbP)
- Characteristics
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- Recombinant human Histone H3.2 (N-term HIS tag) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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Histone H3.2 (biotinylated), (full length), (N-Term), (truncated) protein
Origin: Human Host: Escherichia coli (E. coli) Recombinant Protein was determined to have ≥ 98 % purity by SDS-PAGE. PC, S
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Histone H3.2
- Synonyms
- H3-I Protein, H3-II Protein, H3-III Protein, H3-IV Protein, H3-V Protein, H3-VI Protein, H3-VII Protein, H3-VIII Protein, H3F3A Protein, H3C2 Protein, H3C3 Protein, H3 histone, family 3C Protein, histone H3.2 Protein, Histone H3.2 Protein, H3F3C Protein, LOC504599 Protein, PVX_082615 Protein, Tsp_16072 Protein, H3C4 Protein, LOC106362349 Protein
- Background
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated this record represents the telomeric copy.
- Molecular Weight
- 15.2 kDa
- NCBI Accession
- NP_066403
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