HIST1H2BM Protein (Myc-DYKDDDDK Tag)
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- Target See all HIST1H2BM products
- HIST1H2BM (Histone H2B Type 1-M)
- Protein Type
- Recombinant
- Origin
- Human
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HIST1H2BM protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Histone H2B type 1-M protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- HIST1H2BM (Histone H2B Type 1-M)
- Abstract
- HIST1H2BM Products
- Synonyms
- H2B/e Protein, H2BFE Protein, dJ160A22.3 Protein, H2b-291b Protein, histone cluster 1 H2B family member m Protein, histone cluster 1, H2bm Protein, HIST1H2BM Protein, Hist1h2bm Protein
- Background
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
- Molecular Weight
- 13.8 kDa
- NCBI Accession
- NP_003512
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