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E2F5 Protein (E2F5) (His tag)

E2F5 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN2719897
  • Target See all E2F5 Proteins
    E2F5
    Protein Type
    Recombinant
    Origin
    • 3
    • 1
    Human
    Source
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This E2F5 protein is labelled with His tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human E2F5 (N-term HIS tag, transcript variant 1) protein expressed in E. coli.
    • Produced with end-sequenced ORF clone
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
    Top Product
    Discover our top product E2F5 Protein
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the N-terminal.

    Restrictions
    For Research Use only
  • Concentration
    50 μg/mL
    Buffer
    25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    E2F5
    Alternative Name
    e2f5 (E2F5 Products)
    Synonyms
    AU024671 Protein, E2F-5 Protein, E2f-5 Protein, E2F4 Protein, cb735 Protein, zgc:111879 Protein, E2F transcription factor 5 Protein, E2f5 Protein, e2f5 Protein, E2F5 Protein
    Background
    The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.
    Molecular Weight
    37.4 kDa
    NCBI Accession
    NP_001942
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