DLAT Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719517

Product Details

Target: DLAT (Dihydrolipoyl Transacetylase (DLAT))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This DLAT protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human DLAT / DLTA protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DLAT (Dihydrolipoyl Transacetylase (DLAT))

Alternative Name: Dlat,dlta (DLAT Products)

Synonyms: DLAT Protein, Dmel\\CG5261 Protein, EP(2)0816 Protein, EP816 Protein, anon-WO0118547.121 Protein, DLTA Protein, PDC-E2 Protein, PDCE2 Protein, wu:fc14f10 Protein, wu:fc21f08 Protein, wu:fc86g11 Protein, wu:fj57d06 Protein, 6332404G05Rik Protein, midline uncoordinated Protein, dihydrolipoamide S-acetyltransferase L homeolog Protein, dihydrolipoamide S-acetyltransferase Protein, dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) Protein, muc Protein, dlat.L Protein, DLAT Protein, Dlat Protein, dlat Protein

Background: This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95 % of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009].

Molecular Weight: 59.6 kDa

NCBI Accession: NP_001922