DGKI Protein (Myc-DYKDDDDK Tag)
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- Target See all DGKI products
- DGKI (Diacylglycerol Kinase, iota (DGKI))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DGKI protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human DAG kinase iota protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- DGKI (Diacylglycerol Kinase, iota (DGKI))
- Alternative Name
- Dag Kinase iota (DGKI Products)
- Synonyms
- DGK-IOTA Protein, rDGKi-3 Protein, C130010K08Rik Protein, diacylglycerol kinase iota Protein, diacylglycerol kinase, iota Protein, DGKI Protein, dgki Protein, LOC100560932 Protein, Dgki Protein
- Background
- This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.
- Molecular Weight
- 116.8 kDa
- NCBI Accession
- NP_004708
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