BRAF Protein (DYKDDDDK Tag)
-
- Target See all BRAF Proteins
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This BRAF protein is labelled with DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
-
- Recombinant human B-Raf proto-oncogene protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- BRAF kinase activity was measured in an HTRF® assay. Varying concentrations of BRAF were added to a reaction mix containing ATP and a biotinylated kinase substrate (HTRF substrate 2) and was incubated at 37C for phosphorylation. HTRF detection reagents were then added, the reaction was incubated for 30 minutes at room temperature. Time-resolved fluorescent signal (Delta R) was measured on a Flexstation 3 microplate reader.
- Top Product
- Discover our top product BRAF Protein
-
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- > 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. < td valign=top>
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
- Alternative Name
- B-Raf Proto-Oncogene (BRAF Products)
- Synonyms
- B-RAF1 Protein, BRAF1 Protein, NS7 Protein, RAFB1 Protein, C-RMIL Protein, RMIL Protein, B-Raf Protein, braf1 Protein, rafb1 Protein, b-raf1 Protein, sierrabraf Protein, BRAF Protein, 9930012E13Rik Protein, AA120551 Protein, AA387315 Protein, AA473386 Protein, B-raf Protein, Braf-2 Protein, Braf2 Protein, C230098H17 Protein, C87398 Protein, D6Ertd631e Protein, braf Protein, fc48g01 Protein, wu:fc48g01 Protein, B-Raf proto-oncogene, serine/threonine kinase Protein, B-Raf proto-oncogene, serine/threonine kinase S homeolog Protein, Braf transforming gene Protein, si:dkey-222f8.3 Protein, BRAF Protein, Braf Protein, braf.S Protein, braf Protein, si:dkey-222f8.3 Protein
- Background
- This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
- Molecular Weight
- 44 kDa
- NCBI Accession
- NP_004324
- Pathways
- MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
-