APOE Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714829

Product Details

Target: APOE (Apolipoprotein E (APOE))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This APOE protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Apolipoprotein E / Apo E protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Pan, Zhou, Mahsut, Rohm, Berejnaia, Price, Chen, Castro-Perez, Lassman, McLaren, Conway, Jensen, Thomas, Reyes-Soffer, Ginsberg, Gutstein, Cleary, Previs, Roddy: "Static and turnover kinetic measurement of protein biomarkers involved in triglyceride metabolism including apoB48 and apoA5 by LC/MS/MS." in: Journal of lipid research, Vol. 55, Issue 6, pp. 1179-87, (2016) (PubMed).

Target Details

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: Apolipoprotein E,apo E (APOE Products)

Synonyms: ad2 Protein, apoprotein Protein, im:7036787 Protein, wu:fb69a05 Protein, zgc:110064 Protein, apoe Protein, AI255918 Protein, AD2 Protein, LDLCQ5 Protein, LPG Protein, APOEA Protein, Apo-E Protein, apolipoprotein E Protein, apolipoprotein Ea Protein, apoe Protein, apoea Protein, Apoe Protein, APOE Protein

Background: The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.

Molecular Weight: 34.2 kDa

NCBI Accession: NP_000032

Pathways: Regulation of Cell Size, Lipid Metabolism