APEX1 Protein (Transcript Variant 1)

Catalog No. ABIN2714788

Product Details

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human APEX1 / REF-1 (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 10 mM HEPES, 100 mM KCl, 50 % Glycerol, pH 7.4. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: Apex1,ref-1 (APEX1 Products)

Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene all encode the same protein.

Molecular Weight: 35.6 kDa

NCBI Accession: NP_001632

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus