Acetylcholinesterase Protein (AChE) (Myc-DYKDDDDK Tag)
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- Target See all Acetylcholinesterase (AChE) Proteins
- Acetylcholinesterase (AChE)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Acetylcholinesterase protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Acetylcholinesterase (transcript variant E4-E5) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product AChE Protein
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Acetylcholinesterase (AChE) (AA 1-640) protein (GST tag)
AChE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Acetylcholinesterase (AChE) (AA 32-614) protein (His tag)AChE Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Acetylcholinesterase (AChE) (AA 515-614) protein (GST tag)AChE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Acetylcholinesterase (AChE) (Active) protein (His tag)AChE Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Acetylcholinesterase (AChE) (AA 31-613) protein (GST tag)AChE Origin: Cow Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Acetylcholinesterase (AChE)
- Alternative Name
- Acetylcholinesterase (AChE Products)
- Synonyms
- ACEE Protein, ARACHE Protein, N-ACHE Protein, YT Protein, Ache Protein, GB14873 Protein, zgc:92550 Protein, ACE Protein, Dsim\\GD20515 Protein, GD20515 Protein, dsim_GLEANR_4292 Protein, ache Protein, mE1a Protein, mE1b Protein, mE1c Protein, mE1c-long Protein, mE1d Protein, mE1d' Protein, mE1e Protein, arache Protein, n-ache Protein, acetylcholinesterase (Cartwright blood group) Protein, acetylcholinesterase 2 Protein, acetylcholinesterase Protein, Acetylcholine esterase Protein, acetylcholinesterase (Cartwright blood group) L homeolog Protein, collagen type I alpha 2 chain Protein, ACHE Protein, AChE-2 Protein, Ache Protein, ache Protein, Dsim\Ace Protein, ache.L Protein, COL1A2 Protein, ACE-1 Protein
- Background
- Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.
- Molecular Weight
- 60.8 kDa
- NCBI Accession
- NP_056646
- Pathways
- Skeletal Muscle Fiber Development
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