HOGA1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all HOGA1 Proteins
- HOGA1 (4-Hydroxy-2-Oxoglutarate Aldolase 1 (HOGA1))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HOGA1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Chromosome 10 open reading frame 65 (C10orf65), transcript variant 1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- HOGA1 (4-Hydroxy-2-Oxoglutarate Aldolase 1 (HOGA1))
- Alternative Name
- Chromosome 10 Open Reading Frame 65 (c10orf65) (HOGA1 Products)
- Synonyms
- C10orf65 Protein, DHDPS2 Protein, DHDPSL Protein, HP3 Protein, NPL2 Protein, 0610010D20Rik Protein, Dhdpsl Protein, Npl2 Protein, 4-hydroxy-2-oxoglutarate aldolase 1 Protein, HOGA1 Protein, Hoga1 Protein
- Background
- The authors of PMID:20797690 cloned this gene while searching for genes in a region of chromosome 10 linked to primary hyperoxalurea type III. They noted that even though the encoded protein has been described as a mitochondrial dihydrodipicolinate synthase-like enzyme, it shares little homology with E. coli dihydrodipicolinate synthase (Dhdps), particularly in the putative substrate-binding region. Moreover, neither lysine biosynthesis nor sialic acid metabolism, for which Dhdps is responsible, occurs in vertebrate mitochondria. They propose that this gene encodes mitochondrial 4-hydroxyl-2-oxoglutarate aldolase (EC 4.1.3.16), which catalyzes the final step in the metabolic pathway of hydroxyproline, releasing glyoxylate and pyruvate. This gene is predominantly expressed in the liver and kidney, and mutations in this gene are found in patients with primary hyperoxalurea type III. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
- Molecular Weight
- 35.1 kDa
- NCBI Accession
- NP_612422
- Pathways
- Monocarboxylic Acid Catabolic Process
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