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CH25H Protein (Myc-DYKDDDDK Tag)

CH25H Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN2713950
  • Target See all CH25H Proteins
    CH25H (Cholesterol 25-Hydroxylase (CH25H))
    Protein Type
    Recombinant
    Origin
    • 6
    • 1
    Human
    Source
    • 4
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This CH25H protein is labelled with Myc-DYKDDDDK Tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human CH25H protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
    Top Product
    Discover our top product CH25H Protein
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Concentration
    50 μg/mL
    Buffer
    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    CH25H (Cholesterol 25-Hydroxylase (CH25H))
    Alternative Name
    Ch25h (CH25H Products)
    Synonyms
    CH25H Protein, zgc:101688 Protein, MGC143045 Protein, C25H Protein, AI462618 Protein, m25OH Protein, cholesterol 25-hydroxylase Protein, cholesterol 25-hydroxylase L homeolog Protein, CH25H Protein, ch25h Protein, Ch25h Protein, ch25h.L Protein
    Background
    This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
    Molecular Weight
    31.6 kDa
    NCBI Accession
    NP_003947
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