CH25H Protein (Myc-DYKDDDDK Tag)
-
- Target See all CH25H Proteins
- CH25H (Cholesterol 25-Hydroxylase (CH25H))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CH25H protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human CH25H protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product CH25H Protein
-
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- CH25H (Cholesterol 25-Hydroxylase (CH25H))
- Alternative Name
- Ch25h (CH25H Products)
- Synonyms
- CH25H Protein, zgc:101688 Protein, MGC143045 Protein, C25H Protein, AI462618 Protein, m25OH Protein, cholesterol 25-hydroxylase Protein, cholesterol 25-hydroxylase L homeolog Protein, CH25H Protein, ch25h Protein, Ch25h Protein, ch25h.L Protein
- Background
- This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
- Molecular Weight
- 31.6 kDa
- NCBI Accession
- NP_003947
-