CA5B Protein
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- Target See all CA5B Proteins
- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Carbonic anhydrase 5B protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
- Top Product
- Discover our top product CA5B Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 20 mM Tris-HCl, 100 mM NaCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
- Alternative Name
- Carbonic Anhydrase 5b (CA5B Products)
- Background
- Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.
- Molecular Weight
- 33.8 kDa
- NCBI Accession
- NP_009151
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