CA5B Protein

Catalog No. ABIN2713133

Product Details

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Carbonic anhydrase 5B protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Alternative Name: Carbonic Anhydrase 5b (CA5B Products)

Synonyms: CA-VB Protein, 7330410H16Rik Protein, CAVB Protein, Ca5b Protein, CarVb Protein, D730005F19Rik Protein, zgc:171653 Protein, CA5B Protein, ca5b Protein, carbonic anhydrase 5B Protein, carbonic anhydrase 5b, mitochondrial Protein, carbonic anhydrase Va Protein, carbonic anhydrase 5A Protein, carbonic anhydrase 5B, mitochondrial Protein, carbonic anhydrase VB, mitochondrial L homeolog Protein, CA5B Protein, Car5b Protein, Ca5b Protein, ca5a Protein, CA5A Protein, LOC100051095 Protein, ca5b.L Protein

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.

Molecular Weight: 33.8 kDa

NCBI Accession: NP_009151