Calpain 2 Protein (CAPN2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2713061

Product Details

Target: Calpain 2 (CAPN2)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Calpain 2 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Calpain-2 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Pan, Zhou, Mahsut, Rohm, Berejnaia, Price, Chen, Castro-Perez, Lassman, McLaren, Conway, Jensen, Thomas, Reyes-Soffer, Ginsberg, Gutstein, Cleary, Previs, Roddy: "Static and turnover kinetic measurement of protein biomarkers involved in triglyceride metabolism including apoB48 and apoA5 by LC/MS/MS." in: Journal of lipid research, Vol. 55, Issue 6, pp. 1179-87, (2016) (PubMed).

Miyaji, Shahrizaila, Umapathi, Chan, Hirata, Yuki: "Are ERM (ezrin/radixin/moesin) proteins targets for autoantibodies in demyelinating neuropathies?" in: Human immunology, Vol. 75, Issue 11, pp. 1089-91, (2015) (PubMed).

Oh, Choung, Lee, Park, Lee, Lee, Seo, Park: "CPNE7, a preameloblast-derived factor, regulates odontoblastic differentiation of mesenchymal stem cells." in: Biomaterials, Vol. 37, pp. 208-17, (2015) (PubMed).

Adini, Adini, Bazinet, Watnick, Bielenberg, DAmato: "Melanocyte pigmentation inversely correlates with MCP-1 production and angiogenesis-inducing potential." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, (2014) (PubMed).

Boxer, Barajas, Tao, Zhang, Khavari: "ZNF750 interacts with KLF4 and RCOR1, KDM1A, and CTBP1/2 chromatin regulators to repress epidermal progenitor genes and induce differentiation genes." in: Genes & development, Vol. 28, Issue 18, pp. 2013-26, (2014) (PubMed).

Briz, Hsu, Li, Lee, Bi, Baudry: "Calpain-2-mediated PTEN degradation contributes to BDNF-induced stimulation of dendritic protein synthesis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 10, pp. 4317-28, (2013) (PubMed).

Wang, Briz, Chishti, Bi, Baudry: "Distinct roles for μ-calpain and m-calpain in synaptic NMDAR-mediated neuroprotection and extrasynaptic NMDAR-mediated neurodegeneration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 48, pp. 18880-92, (2013) (PubMed).

Ye, Gao, Tian, Yearsley, Lange, Robertson, Barsky: "Early to intermediate steps of tumor embolic formation involve specific proteolytic processing of E-cadherin regulated by Rab7." in: Molecular cancer research : MCR, Vol. 10, Issue 6, pp. 713-26, (2012) (PubMed).

Lin, Wu, Dong, Bouck, Zeng, Chen: "Cyclin-dependent kinase 2 negatively regulates human pregnane X receptor-mediated CYP3A4 gene expression in HepG2 liver carcinoma cells." in: The Journal of biological chemistry, Vol. 283, Issue 45, pp. 30650-7, (2008) (PubMed).

Target Details

Target: Calpain 2 (CAPN2)

Alternative Name: Calpain-2 (CAPN2 Products)

Synonyms: CANP2 Protein, CANPL2 Protein, CANPml Protein, mCANP Protein, AI326419 Protein, Capa-2 Protein, Capa2 Protein, m-calpain Protein, m-calpin Protein, capn2 Protein, CAPN2 Protein, im:6908877 Protein, zgc:109906 Protein, calpain 2 Protein, calpain 2, gene2 Protein, calpain 2, (m/II) large subunit b Protein, CAPN2 Protein, Capn2 Protein, capn2.2 Protein, capn2b Protein

Background: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 79.8 kDa

NCBI Accession: NP_001739