KDM6A Protein (DYKDDDDK Tag)
Quick Overview for KDM6A Protein (DYKDDDDK Tag) (ABIN2669708)
Target
See all KDM6A ProteinsProtein Type
Origin
Source
Application
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Purification tag / Conjugate
- This KDM6A protein is labelled with DYKDDDDK Tag.
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Characteristics
- Recombinant UTX / KDM6A (accession number NP_066963.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 158.8 kDa.
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Lysine (K)-Specific Demethylase 6A (KDM6A) (AA 1-1401) protein (His tag)
custom-made KDM6A Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysine (K)-Specific Demethylase 6A (KDM6A) (AA 1-1401) protein (His tag)custom-made KDM6A Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysine (K)-Specific Demethylase 6A (KDM6A) (AA 641-740) protein (GST tag)KDM6A Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Lysine (K)-Specific Demethylase 6A (KDM6A) protein (Myc-DYKDDDDK Tag)KDM6A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Recombinant UTX / KDM6A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K27me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant UTX / KDM6A protein, and 3.3 μM H3K27me3 peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- KDM6A (Lysine (K)-Specific Demethylase 6A (KDM6A))
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Alternative Name
- UTX / KDM6A
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Background
- KDM6A (lysine (K)-specific demethylase 6A), also known as UTX (Ubiquitously Transcribed Tetratricopeptide Repeat, X Chromosome) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 27 residues of histone H3, while it has no activity on monomethylated H3K27 residues. KDM6A plays a central role in posterior development by regulating HOX gene expression. Demethylation of lysine 27 is concomitant with methylation of lysine 4 of histone H3 and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.
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Molecular Weight
- 158.8 kDa
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Pathways
- Tube Formation, Warburg Effect
Target
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