KDM3B Protein (DYKDDDDK Tag)
KDM3B
Origin: Human
Host: Baculovirus
Recombinant
EAA, ScA
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- Target See all KDM3B Proteins
- KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- Baculovirus
- Purification tag / Conjugate
- This KDM3B protein is labelled with DYKDDDDK Tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant JMJD1B / KDM3B (accession number NP_057688.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with a molecular weight of 193.4 kDa.
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- Discover our top product KDM3B Protein
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Lysine (K)-Specific Demethylase 3B (KDM3B) (AA 1-759) protein (GST tag)
KDM3B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Application Notes
- Recombinant JMJD1B / KDM3B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me2 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 40 nM Recombinant JMJD1B / KDM3B protein, and 3.3 μM H3K9me2 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))
- Alternative Name
- JMJD1B / KDM3B (KDM3B Products)
- Synonyms
- KDM3B Protein, JMJD1B Protein, 5830462I21Rik Protein, JHDM2B Protein, Jmjd1b Protein, mKIAA1082 Protein, 5qNCA Protein, C5orf7 Protein, NET22 Protein, lysine demethylase 3B Protein, lysine (K)-specific demethylase 3B Protein, lysine-specific demethylase 3B Protein, KDM3B lysine (K)-specific demethylase 3B Protein, KDM3B Protein, LOC100540242 Protein, kdm3b Protein, Kdm3b Protein
- Background
- Lysine (K)-specific demethylase 3B (KDM3B), also known as Jumonji domain-containing protein 1B (JMJD1B), is a histone demethylase that specifically demethylates lysine 9 of histone H3 (H3K9), thereby playing a central role in defining the histone code. Demethylation of lysine residues generates formaldehyde and succinate. KDM3B may have tumor suppressor activity.
- Molecular Weight
- 193.4 kDa
- Pathways
- Warburg Effect
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