Histone H4 Protein (HISTH4) (H4K20me3, pseudo-methylated) (His tag)

Catalog No. ABIN2669514

Recombinant Histone H4 protein expressed in Escherichia coli (E. coli).

Quick Overview for Histone H4 Protein (HISTH4) (H4K20me3, pseudo-methylated) (His tag) (ABIN2669514)

Target: Histone H4 (HISTH4)

Protein Type: Recombinant

Origin: Xenopus laevis

Source: Escherichia coli (E. coli)

Application: Positive Control (PC), Substrate (S)

Purity: >98 % pure by SDS-PAGE

Product Details

Protein Characteristics: H4K20me3, pseudo-methylated

Purification tag / Conjugate: This Histone H4 protein is labelled with His tag.

Purpose: Recombinant Histone H4K20me3 (MLA)

Characteristics: Recombinant Xenopus laevis Histone H4 trimethyl Lys20 (H4K20me3) is produced in E. coli and purified using FPLC. Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). This specific chemical treatment enables the site and degree of methylation to be controlled precisely. Each methylation reaction is over 99 % complete, as verified by high-resolution ESI-TOF mass spectrometry. Protein concentration was determined using the molar extinction coefficient for Histone H4 and absorbance at 280nm. The recombinant histone is >98 % pure by SDS-PAGE. The molecular weight of the recombinant histone is 11,297 Daltons. Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Recombinant histones are used to analyze histone modifications, or to generate chromatin in vitro.

Purification: purified using FPLC.

Application Details

Application Notes: Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

Comment:

Pseudomethylated Lysines have been generated using the partented Methylated Lysin Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine gamma-methylene. The MLA technique provides precise control over the site and degree of methylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/mL. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.

Buffer: 50 μg supplied as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/mL. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -20 °C,-80 °C

Storage Comment: Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4

Background:

Protein Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

Short Description: Recombinant Xenopus laevis Histone H4 trimethyl Lys20 (H4K20me3) is produced in E. coli and purified using FPLC. Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). This specific chemical treatment enables the site and degree of methylation to be controlled precisely. Each methylation reaction is over 99 % complete, as verified by high-resolution ESI-TOF mass spectrometry. Protein concentration was determined using the molar extinction coefficient for Histone H4 and absorbance at 280nm. The recombinant histone is >98 % pure by SDS-PAGE. The molecular weight of the recombinant histone is 11,297 Daltons.

Molecular Weight: 11,297 Daltons