IL-9 Protein (AA 19-144)
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- Target See all IL-9 (IL9) Proteins
- IL-9 (IL9) (Interleukin 9 (IL9))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 19-144
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC)
- Purity
- >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Endotoxin Level
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Less than 0.1 ng per μg of protein.
- Top Product
- Discover our top product IL9 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Biological activity: ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5.0 x 106 units/mg as determined by the dose-dependent stimulation of the proliferation of human M07e cells.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
- Buffer
- Lyophilized
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Unopened vial can be stored at -20°C or -70°C.
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- Target
- IL-9 (IL9) (Interleukin 9 (IL9))
- Alternative Name
- IL-9 (IL9 Products)
- Synonyms
- IL9 Protein, IL-9 Protein, Il-9 Protein, P40 Protein, HP40 Protein, interleukin 9 Protein, IL9 Protein, il9 Protein, Il9 Protein
- Background
- IL-9 was initially identified in mice as a T cell growth factor. Human and murine IL-9 show a 56 % amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα ) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15, and IL-21. IL-9 is expressed by activated Th9, Th17, Tregs, and mast cells and appears to play an important role in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immunosuppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a key factor in the pathogenesis of bronchial hyperresponsiveness. In this sense, it has been described that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability and plays a role in the development of food allergies. IL-9 has also been associated with atopic dermatitis.
- Molecular Weight
- The 127 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 14.2 kDa. The predicted N-terminal amino acid is Met.
- Pathways
- JAK-STAT Signaling
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