IL-22 Protein (AA 34-179)
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- Target See all IL-22 (IL22) Proteins
- IL-22 (IL22) (Interleukin 22 (IL22))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 34-179
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Western Blotting (WB), ELISA
- Purity
- Purity is >98 % , as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
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Endotoxin level is <0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.
- Top Product
- Discover our top product IL22 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.
- Buffer
- Lyophilized in sterile-filtered PBS, pH 7.2, containing 1 % BSA and protease inhibitors.
- Handling Advice
- Use a manual defrost freezer and avoid repeated freeze thaw cycles.
- Storage
- 4 °C
- Storage Comment
- Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- IL-22 (IL22) (Interleukin 22 (IL22))
- Alternative Name
- IL-22 (IL22 Products)
- Synonyms
- IL-21 Protein, IL-22 Protein, IL-D110 Protein, IL-TIF Protein, ILTIF Protein, TIFIL-23 Protein, TIFa Protein, zcyto18 Protein, IL-22a Protein, ILTIFa Protein, Iltif Protein, RGD1561292 Protein, interleukin-22 Protein, interleukin 22 Protein, IL22 Protein, il22 Protein, Il22 Protein
- Background
- IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29. Human IL-22 shares 79 % amino acid identity with murine IL-22 and 25 % identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.
- Molecular Weight
- The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16.88 kDa. The DTT-reduced protein migrates at approximately 15 kDa and the non-reduced protein migrates at approximately 14 kDa by SDS-PAGE.
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