IGF2 Protein (AA 25-91)
-
- Target See all IGF2 Proteins
- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 25-91
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Application
- Immunoprecipitation (IP), Flow Cytometry (FACS)
- Purity
- >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Endotoxin Level
-
Less than 0.1 ng per μg of protein.
- Top Product
- Discover our top product IGF2 Protein
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Biological activity: ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5.0 x 105 units/mg as determined by its ability to stimulate the proliferation of mouse FDC-P1 cells.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
- Buffer
- Lyophilized, carrier-free.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Unopened vial can be stored at -20°C or -70°C.
-
- Target
- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
- Alternative Name
- IGF-II (IGF2 Products)
- Synonyms
- C11orf43 Protein, IGF-II Protein, PP9974 Protein, LOC100136550 Protein, igf2 Protein, igf2b Protein, wu:fc26e03 Protein, zgc:91781 Protein, igf-2 Protein, insigf Protein, pp9974 Protein, AL033362 Protein, Igf-2 Protein, Igf-II Protein, M6pr Protein, Mpr Protein, Peg2 Protein, IGFII Protein, RNIGF2 Protein, IGF-II-B Protein, igf2-a Protein, igf2-b Protein, insulin like growth factor 2 Protein, insulin-like growth factor II Protein, insulin-like growth factor 2a Protein, insulin like growth factor 2 S homeolog Protein, insulin-like growth factor 2 Protein, insulin-like growth factor 2 (somatomedin A) Protein, insulin like growth factor 2 L homeolog Protein, IGF2 Protein, LOC100136550 Protein, igf2a Protein, igf2.S Protein, Igf2 Protein, igf2.L Protein
- Background
- IGF-II is a member of the IGF family, comprised of three members (IGF-I, IGF-II, and insulin). IGF-I and IGF-II share approximately 50 % similarity with insulin at the amino acid level. IGF-II is synthesized as a 180 amino acid prepro-IGF-II, pro-IGF-II is processed to its mature, active protein form by glucose regulating protein 94 (GRP94), which possesses chaperone activity towards IGF-II. GRP94 and IGF-II play a key role in muscle differentiation. Seven binding proteins have been described (IGFBP-1 to -7). IGFBPs extend the half life and regulate the availability of the IGFI and IGFII. It has been described that IGFBP3 is the main circulating binding protein for IGFs, in serum, approximately 75 % of circulating IGF-I and IGF-II bind to IGFBP3 and the co-carrier acid labile subunit (ALS). IGF-II binds to three receptors, and the binding to IGFIR promotes cell proliferation, survival, differentiation, and migration as well as metabolic function of IGF-II. Embryonic stem cell differentiation into endothelial cells is promoted by the binding of IGF-II to IGFR1. IGF-II binding to IGF-IIR induces internalization and clearing of IGF-II. Osteogenic differentiation and bone formation is enhanced by IGF-II through bone morphogenetic protein-9 (BMP-9). Polymorphisms of IGF-II are associated with obesity, cardiovascular risk factors, and hypertension.
- Molecular Weight
- The 67 amino acid recombinant protein has a predicted molecular mass of approximately 7.5 kDa. The N-terminal amino acid is Ala.
- Pathways
- Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
-