FGF8 Protein (AA 23-215, N-Term)
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- Target See all FGF8 Proteins
- FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 23-215, N-Term
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Flow Cytometry (FACS)
- Purity
- >97 % , as determined by Coomassie stained SDS-PAGE gel and HLPC analysis.
- Endotoxin Level
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Less than 0.1 ng per μg of protein.
- Top Product
- Discover our top product FGF8 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Biological activity: ED50 = 2.0 - 4.0 ng/ml, corresponding to a specific activity of 2.5 - 5.0 x 105 units/mg,as measured by its ability to stimulate proliferation of BALB/c 3T3 cells in a dose dependent manner.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
- Buffer
- Lyophilized, carrier-free.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Unopened vial can be stored at -20°C or -70°C.
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- Target
- FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))
- Alternative Name
- FGF-8 (FGF8 Products)
- Synonyms
- Aigf Protein, Fgf-8 Protein, AIGF Protein, FGF-8 Protein, HBGF-8 Protein, HH6 Protein, KAL6 Protein, fibroblast growth factor 8 Protein, Fgf8 Protein, FGF8 Protein
- Background
- FGF-8 belongs to the fibroblast growth factor (FGF) family and plays an important role in cell growth, embryogenesis, and tumorigenesis. Four isoforms (a, b, e, and f) of human FGF-8 generated by alternative splicing of the mRNA have been described. The isoforms may have different functions during embryogenesis. During prenatal stages, FGF-8 is required for the normal development of various organs, including limbs and central nervous system. FGF-8 was first cloned from SC-3 mouse mammary carcinoma cells and was found to be induced in response to androgenic stimuli. FGF-8 is found to have a transforming capacity and is detected in human prostate and breast carcinoma specimens and cell lines. The isoform FGF-8b has the highest affinity to FGF receptors and the strongest transforming capacity.
- Molecular Weight
- The 194 amino acid recombinant protein has a predicted molecular mass of approximately 22.5 kDa. The predicted N-terminal amino acid is Met.
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis
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