Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Cathepsin E Protein (CTSE) (AA 18-396)

CTSE Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % , as determined by Coomassie stained SDS-PAGE. ICFC Active
Catalog No. ABIN2667383
  • Target See all Cathepsin E (CTSE) Proteins
    Cathepsin E (CTSE)
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 18-396
    Origin
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 6
    • 3
    • 3
    • 3
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    Intracellular Flow Cytometry (ICFC)
    Purity
    >95 % , as determined by Coomassie stained SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level

    Less than 0.01 ng per μg cytokine as determined by the LAL method.

    Top Product
    Discover our top product CTSE Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Biological activity: After auto-activation, CTSE activity is determined by its ability to cleave the fluorogenic peptide substrate, Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2. The specific activity is >1,500 pmol/min/μg.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    For maximum results, quick spin vial prior to opening.
    Buffer
    0.22 μm filtered protein solution is in 20 mM MES, 150 mM NaCl, pH 6.5.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Unopened vial can be stored at -70°C for six months.
  • Target
    Cathepsin E (CTSE)
    Alternative Name
    Cathepsin E (CTSE Products)
    Synonyms
    CATE Protein, A430072O03Rik Protein, C920004C08Rik Protein, CE Protein, CatE Protein, CEA Protein, CEB Protein, Ctsea Protein, CTSE Protein, ce1 Protein, ce2 Protein, cate Protein, CE1 Protein, ce1-A Protein, ctse Protein, CE2 Protein, ce2-A Protein, cathepsin E Protein, cathepsin E L homeolog Protein, cathepsin E S homeolog Protein, CTSE Protein, Ctse Protein, TP03_0056 Protein, Smp_184930 Protein, ctse Protein, ctse.L Protein, ctse.S Protein
    Background
    Cathepesin E (CTSE) is an intracellular aspartic protease that was originally identified as a cathepsin D-like acid protease. CTSE and CTSD have similar substrate specificities and CTSE is active in acidic conditions in a pH range from 2.5 to 5.5. In vitro experiments have identified several CTSE substrates including insulin beta chain, neurokinin, and FGF. Although the function of CTSE is not completely understood, it has been implicated in several physiological and pathological processes. CTSE is required for antigen presentation on class II MHC molecules and subsequently, CTSE-deficient mice have increased susceptibility to bacterial infections. CTSE-deficient macrophages also show abnormalities, such as autophagy. Like many other cathepsins, CTSE has emerged as a therapy target for cancers, such as pancreatic ductal adenocarcinoma (PDAC). In addition to PDAC, CTSE is also overexpressed in gastric carcinomas and cervical and lung adenocarcinomas. The possible involvement of CTSE in neurodegeneration has also been reported.
    Molecular Weight
    The 389 amino acid recombinant protein has a predicted molecular mass of approximately 42.3 kDa. The DTT-reduced protein migrates at approximately 45 kDa and the non-reduced protein migrates at approximately 90 kDa by SDS-PAGE. The N-terminal amino acid i
You are here:
Support