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Ccl12 Protein (AA 23-104)

Ccl12 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 98 % , as determined by Coomassie stained SDS-PAGE. FACS Active
Catalog No. ABIN2666885
  • Target See all Ccl12 Proteins
    Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 23-104
    Origin
    Mouse
    Source
    • 4
    Escherichia coli (E. coli)
    Application
    Flow Cytometry (FACS)
    Purity
    > 98 % , as determined by Coomassie stained SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level

    Less than 0.01 ng per μg cytokine as determined by the LAL method.

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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Biological activity: Bioactivity was measured by its ability to chemoattract THP-1 cells in a dose dependent manner.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
    Buffer
    0.22 μm filtered protein solution is in PBS.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
  • Target
    Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
    Alternative Name
    CCL12 (Ccl12 Products)
    Synonyms
    MCP-5 Protein, Scya12 Protein, chemokine (C-C motif) ligand 12 Protein, Ccl12 Protein
    Background
    Mouse CCL12 belongs to the CC chemokine family and is most closely related to human CCL2 (MCP-1) in structure (66 % amino acid identity). CCL12 binds to cell surface receptor CCR2, a human CCL2 primary receptor, and can induce migration and Ca2+ flux. CCL12 is a potent chemoattractant for peripheral blood monocytes but only has weak activity on eosinophils at high doses. CCL12 is produced by macrophages during early allergic reactions and infections. During murine MCV infection, bone marrow F4/80 (+) cells can produce high levels of CCL12. The expression of CCL12 can also be upregulated by bacterial infections including pneumococcal meningitis and polymicrobial septic peritonitis. LPS can induce the expression of CCL12 in liver macrophages (Kupffer cells) in vivo. CCL12 is involved in the pathogenesis of pulmonary fibrosis by recruiting fibrocytes to the injury site and enhancing fibrotic responses. Also, CCL12 is implicated in liver and kidney fibrosis. It has also been shown that hypoxia inducible factor 1 is involved in hypoxia-induced upregulation of CCL12 in astrocytes. In addition, CCL12 is involved in joint and growth plate development.
    Molecular Weight
    The 82 amino acid recombinant protein has a predicted molecular mass of approximately 10 kDa. The DTT-reduced protein migrates at approximately 13 kDa and non-reduced protein migrates at approximately 15 kDa by SDS-PAGE. The N-terminal amino acid is Gly.
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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