IL-34 Protein
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- Target See all IL-34 (IL34) Proteins
- IL-34 (IL34) (Interleukin 34 (IL34))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- CHO Cells
- Application
- Flow Cytometry (FACS)
- Sterility
- 0.22 μm filtered
- Top Product
- Discover our top product IL34 Protein
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Interleukin 34 (IL34) protein
IL34 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Interleukin 34 (IL34) protein (Myc-DYKDDDDK Tag)IL34 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- Buffer
- 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 0.5 M NaCl, pH 7.4.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- Target
- IL-34 (IL34) (Interleukin 34 (IL34))
- Alternative Name
- IL-34 (IL34 Products)
- Synonyms
- C16orf77 Protein, IL-34 Protein, 2010004A03Rik Protein, AI593503 Protein, C18H16ORF77 Protein, interleukin 34 Protein, IL34 Protein, Il34 Protein
- Background
- Human IL-34 shares a sequence identity of 99.6 % , 72 % and 71 % with chimpanzee, rat, and mouse IL-34, respectively, on the amino acid level. The IL-34 gene is syntenic in the human, chimpanzee, rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif, and does not share sequence homology with M-CSF, nevertheless, it binds to the CSFR. These two cytokines are not identical in biological activity and signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines have differing ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests a differential domain binding in the receptor to IL-34 and CSF and, as a result, different bioactivities and signal activation kinetics/strength are produced for these cytokines.
- Pathways
- RTK Signaling
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