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VEGF121 Protein (AA 207-327)

VEGF121 Origin: Human Host: HEK-293 Cells Recombinant > 98 % , as determined by Coomassie stained SDS-PAGE. FACS Active
Catalog No. ABIN2666678
  • Target See all VEGF121 Proteins
    VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 207-327
    Origin
    • 13
    • 1
    • 1
    Human
    Source
    • 8
    • 6
    • 1
    HEK-293 Cells
    Application
    Flow Cytometry (FACS)
    Purity
    > 98 % , as determined by Coomassie stained SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level

    Less than 0.01 ng per μg cytokine as determined by the LAL method.

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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Biological activity: ED50 = 0.5 - 2.5 ng/ml, corresponding to a specific activity of 0.4 - 2.0 x 106 units/mg, as determined by the dose dependent stimulation of HUVEC cells proliferation.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
    Buffer
    0.22 μm filtered protein solution is in 5 mM citric acid, 5 mM NaHPO4, 0.15 M NaCl, pH 4.0.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
  • Target
    VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
    Alternative Name
    VEGF-121 (VEGF121 Products)
    Background
    VEGF was initially identified in conditioned medium from bovine pituitary follicular cells. VEGF-A belongs to the VEGF family, which has the following members: VEGF-A, VEGF-B, VEGF-C (VEGF-2), VEGF-D, and PlGF (placental growth factor). In addition, viral VEGF homologs (collectively called VEGF-E) and snake venom VEGFs such as T.f. ( Trimeresurus flavoviridis) and svVEGF (called VEGF-F) have been described. VEGFA is alternatively spliced to generate variants with different number of amino acids such as VEGFA121, VEGFA145, VEGFA165, and VEGFA189. While VEGFA121 is freely diffusible and did not bind to neurophilis (NRPs) nor heparin sulphate, VEGFA165 and VEGFA189 bind to both, resulting in retention on the cell surface or in the extracellular matrix. VEGF-A is highly expressed in solid tumors generated in breast, lung, renal, colorectal and liver tissues. VEGF has strong vascular permeability activity and significantly contributes to the formation of ascites tumors. VEGF can act as a direct proinflammatory mediator during the pathogenesis of rheumatoid arthritis (RA) and protect rheumatoid synoviocytes from apoptosis, which contributes to synovial hyperplasia. VEGF is expressed in synovial macrophages and synovial fibroblasts in RA patients. Also, VEGF is associated with age-related macular degeneration (AMD). AMD is due to neovascularization that originates from endothelial cells in the choroid that grow into neurosensory retina as choroidal neovascularization (CNV).
    Molecular Weight
    The 121 amino acid recombinant protein has a predicted molecular mass of approximately 14 kDa. The DTT-reduced protein migrates at approximately 20 kDa and non-reduced protein migrates at 40 kDa by SDS-PAGE. The N-terminal amino acid is Alanine.
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