CXCL11 Protein (AA 22-94)

Catalog No. ABIN2666634

Product Details

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 22-94

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Bioactivity was measured by its property to chemoattract Baf3-mCXCR3 transfectants in a dose dependent manner. The ED50 = 1 - 5 ng/m L.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Alternative Name: CXCL11 (CXCL11 Products)

Synonyms: H174 Protein, I-TAC Protein, IP-9 Protein, IP9 Protein, SCYB11 Protein, SCYB9B Protein, b-R1 Protein, Cxc11 Protein, I-tac Protein, Ip9 Protein, Itac Protein, Scyb11 Protein, Scyb9b Protein, betaR1 Protein, C-X-C motif chemokine ligand 11 Protein, chemokine (C-X-C motif) ligand 11 Protein, CXCL11 Protein, Cxcl11 Protein

Background: CXCL11 is a CXC chemokine lacking the ELR (Glutamate-Leucine-Arginine) tripeptide motif, which is functionally and structurally related to CXCL9 and CXCL10. CXCL11, together with CXCL9 and CXCL10, specifically binds to the G protein-coupled receptor CXCR3 and is involved in chemotaxis of immune cells and angiogenesis. Expression of both CXCR3 and CXCL11 by the Th1-associated cytokine IFNγ creates an amplification loop of cell-mediated immune response between Th1 cells. CXCR7 has been identified as an alternate receptor for CXCL11 and is involved in growth, survival, and adhesion of tumor cells.

Molecular Weight: The 74 amino acid recombinant protein has a predicted molecular mass of approximately 8.3 kDa. The DTT-reduced protein migrates at approximately 11 kDa and non-reduced protein migrates at approximately 13 kDa by SDS-PAGE. The N-terminal amino acid is Meth