BMP6 Protein (AA 344-460)

Catalog No. ABIN2666465

This Recombinant BMP6 protein is expressed in HEK-293 Cells.

Quick Overview for BMP6 Protein (AA 344-460) (ABIN2666465)

Target: BMP6 (Bone Morphogenetic Protein 6 (BMP6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Multiplex Assay (MA)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Product Details

Protein Characteristics: AA 344-460

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: BMP-6 induces alkaline phosphatase production on ATDC-5 cells. The ED50 = 0.03 - 0.06 μg/ml, corresponding to a specific activity of 1.6 - 3.3 x 104 unit/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: BMP6 (Bone Morphogenetic Protein 6 (BMP6))

Alternative Name: BMP-6

Background: Bone morphogenetic proteins (BMPs) belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I (ALK-1). Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. Human recombinant BMP-6 can restore bone in animal models of osteoporosis. BMP-6 induces bone formation through IGF-I and EGF pathways in a murine model of osteopenia and in human osteoblasts. BMP-6 has been reported to have an antiproliferative effect on B, T cells, and macrophages. Also, BMP-6 induced expression of pro-inflammatory inducible nitric oxide synthase (iNOS), TNF-α and IL-1β in macrophages. In addition, BMP-6 is a key endogenous regulator of hepcidin expression and iron metabolism in vivo. In fact, BMP-6 null mice have a phenotype that resembles mouse models of juvenile hemochromatosis (an iron overload disorder caused by mutations in the genes encoding the major iron regulatory hormone hepcidin and hemojuvelin).

Molecular Weight: The 117 amino acid recombinant protein has a predicted molecular mass of approximately 13 kDa. Recombinant human BMP-6 is a 26.2 kDa homodimeric glycoprotein. The predicted N-terminal amino acid is Val.

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process