IgE Protein

Catalog No. ABIN2648126

Product Details

Target: IgE

Origin: Human

Source: Human

Application: Isotype Control (IsoC)

Characteristics: Purified Human IgE protein
Source: Human myeloma plasma
Alternative Names: Immunoglobulin E Protein, Human IgE

Purification: Purified

Purity: > 95 % pure

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 15 mM KH5 O4, pH 7.4, with 155 M NaCl and 0.05 % NaN3.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

References

Kraus, Kleines, Albers, Blohm, Piechotta, Püttmann, Barth, Nähring, Nebling: "Quantitative measurement of human anti-HCV Core immunoglobulins on an electrical biochip platform." in: Biosensors & bioelectronics, Vol. 26, Issue 5, pp. 1895-901, (2011) (PubMed).

Wang, Munir, Li, Zhou: "Aptamer-Au NPs conjugates-enhanced SPR sensing for the ultrasensitive sandwich immunoassay." in: Biosensors & bioelectronics, Vol. 25, Issue 1, pp. 124-9, (2009) (PubMed).

Cho, Collett, Szafranska, Ellington: "Optimization of aptamer microarray technology for multiple protein targets." in: Analytica chimica acta, Vol. 564, Issue 1, pp. 82-90, (2007) (PubMed).

Olivieri, Beccarini, Gallucci, Romano, Santoro: "Capture assay for specific IgE. An improved quantitative method." in: Journal of immunological methods, Vol. 157, Issue 1-2, pp. 65-72, (1993) (PubMed).

Target Details

Target: IgE

Abstract: IgE Products

Synonyms: IGH2 Protein, IgE Protein, Gm900 Protein, immunoglobulin heavy chain (epsilon polypeptide) Protein, immunoglobulin heavy constant epsilon Protein, Immunoglobulin heavy constant epsilon Protein, Ighe Protein, IGHE Protein

Background: IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 mg/mL of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a myeloma source is a useful protein for studying immunoglobulin behavior.

Molecular Weight: 200 kDa