Streptolysin O Protein (SLO)

Catalog No. ABIN2452205

Product Details

Target: Streptolysin O (SLO)

Protein Type: Recombinant

Biological Activity: Active

Origin: Streptococcus pyogenes

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Characteristics: Measurement of the activity: Definition of 1HU is activation of 50 % hemolysis using 3 % sheep red blood cells.
The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci. The specific activity is as high as 1,900,000 hemolytic units (HU) /mg and the product forms a big hole on the cell membrane, which enables the introduction of protein inside the cells in vivo.

Purity: > 98 % by SDS-PAGE

Sterility: Sterile filtered

Application Details

Application Notes: 1) Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent
2) Reagent for membrane pore formation to introduce small-to-macromolecules into living cells

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Inactivated SLO can be reactivated by thiol reagents such as 20 mM cysteine or 10 mM DTT (2)

Concentration: 1 mg/mL

Buffer: PBS, 1 mM DTT, 50 % glycerol

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References

Maeda, Ide, Koike, Uchiyama, Kinoshita: "GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus." in: Nature cell biology, Vol. 10, Issue 10, pp. 1135-45, (2008) (PubMed).

Walev, Bhakdi, Hofmann, Djonder, Valeva, Aktories, Bhakdi: "Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 6, pp. 3185-90, (2001) (PubMed).

Palmer: "The family of thiol-activated, cholesterol-binding cytolysins." in: Toxicon : official journal of the International Society on Toxinology, Vol. 39, Issue 11, pp. 1681-9, (2001) (PubMed).

Target Details

Target: Streptolysin O (SLO)

Alternative Name: Streptolysin O (SLO Products)

Synonyms: streptolysin O Protein, Streptolysin O Protein, Thiol-activated cytolysin Protein, slo Protein, M6_Spy0188 Protein

Background: Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cells. SLO is easily inactivated in the presence of oxygen but can be reactivated by thiol compounds, so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98 %.